Abstract
Within the last 60 years peptidoglycan (PGN) composition of bacteria has been intensively studied by various methods. However, preparation of pure PGN relied on a very time-consuming method. We established a purification protocol which can be completely performed in plastic reaction tubes or even microtiter plates within 24 hours. The muropeptides can be analyzed by reversed phase UPLC-MS, allowing their immediate determination even in high throughput.
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