Imunolocalização de angiotensina-(1-7), angiotensina II e enzima-conversora de angiotensina 2 em ovário de cabras

Lauro César Soares Feitosa,Adeline Andrade Carvalho,Amilton Paulo Raposo Costa,Kinulpe Honorato-Sampaio,Antônio De Sousa Junior,Alécio Matos Pereira

doi:10.21708/avb.2021.15.3.9629

Abstract

There is increasing evidence as to the participation of the ovarian renin-angiotensin system in important reproductive processes. The inhibition of the angiotensin-converting enzyme (ACE) caused an increase in the rate of ovulation and pregnancy in the artificial insemination protocol has fixed time (TFIA). This study aimed to investigate the presence and location of Ang II, Ang- (1-7) and ACE2 in goat ovaries and the possibility of the involvement of these peptides in previous results. Ten ovaries from goats were collected in a slaughterhouse, washed in buffered PBS, perfused with protease inhibitor solution and processed for immunohistochemistry protocol. The search for peptides was performed using the avidin–biotin–peroxidase method. A strong immunoreactivity for Ang II in theca cells of antral follicles and corpus luteum was observed. Antral follicles (theca cells), corpus luteum and oocyte cytoplasm in early antral follicles exhibited strong immunoreactivity for Ang-(1-7). There was strong immunoreactivity for ACE2 in the cytoplasm of luteal cells and theca cells of antral follicles. In this study, for the first time, the presence and location of Ang II, Ang-(1-7) and ACE2 are reported in goat ovary, suggesting that there is participation in follicular development, oocyte maturation and corpus luteum development.

Highlights

From the discovery of renin to the present days, with the discovery of new components, the renin-angiotensin system (RAS) has been recognized as a regulator of various functions of the animal organism, with emphasis on electrolytic and blood pressure control
Strong immunoreactivity was observed for Ang II, Ang- (1-7) and ACE2 in various ovary compartments, mainly in theca and luteal cells
For Ang-(1-7), strong immunoreactivity was observed in theca cells of antral follicles, corpus luteum and blood vessels, and mild immunoreactivity in the stroma and granulosa cells

Summary

Introduction

From the discovery of renin to the present days, with the discovery of new components, the renin-angiotensin system (RAS) has been recognized as a regulator of various functions of the animal organism, with emphasis on electrolytic and blood pressure control. Mainly because inhibition of the angiotensin-converting enzyme (ACE) increases the bioavailability of Ang- (1-7) (CHAPPELL et al, 1998), an experiment showed an increase in the production of estradiol, close to ovulation, in sheep that used enalapril in the last three days of an FTAI protocol (COSTA et al, 2014) In another experiment, it was demonstrated, in goats, that the addition of the ACE inhibitor (enalapril) to the protocol of artificial insemination in fixed time (IATF), subcutaneously for 12 days, increased the rates of pregnancy, calving and twinning (FERNANDES NETO et al, 2018). The aim of this study was to verify the presence and location of the converting enzyme Ang II, Ang- (1–7) and angiotensin-converting enzyme 2 (ECA2) in goat ovarian tissue

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Conclusion