Abstract
Intermediate-sized non-coding RNAs (imsncRNAs) have been shown to play important regulatory roles in the development of several eukaryotic organisms. In the present research, we selected imsncRNA 761 (imsnc761) as a research target. Expression analyses in a previous study showed that imsnc761 was down-regulated in maturation-arrested testis tissues as compared with the level in normal controls. In the present study, we found that imsnc761 could interact with DEAD-box helicase 6 (DDX6) to induce NTERA-2 (NT2 (testicular embryonal carcinoma cell)) cell apoptosis and proliferation inhibition via the p53 pathway. This interaction between imsnc761 and DDX6 also inhibited mitochondrial function and specific gene transcription and translation. To facilitate further research, we used label-free quantitation method to analyze the associated differences in Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathways and biological processes. This confirmed the changes in several specific pathways, which matched our molecular experimental results.
Highlights
Large-scale studies have clarified that only 2% of all human genome transcripts are translated into proteins [1,2]
The existence of imsncRNAs has been demonstrated but they are yet to be deeply explored. This type of non-coding RNA (ncRNA) can play a variety of roles in an organism, including participating in transcription and translation or interacting with other biological elements to regulate biological functions [40,41,42,43]
Based on sequencing results, we selected to explore the biological function and significance of imsnc761, which is differently expressed in the testes
Summary
Large-scale studies have clarified that only 2% of all human genome transcripts are translated into proteins [1,2]. The limited published data on these molecules suggest that imsncRNAs play specific regulatory roles in the development of several eukaryotic organisms [6,7,8]. They have barely been explored in humans. Imsnc761 is 129 nts long, and its transcription direction is consistent with TPT1 mRNA. It is identified as a small nucleolar RNA, H/ACA box 31B (SNORA31B) [9]
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