Abstract
In this study, peanut, sesame, and rapeseed oil bodies (OBs) were extracted by the aqueous medium method. The surface protein composition, microstructure, average particle size , ζ-potential of the extracted OBs in aqueous emulsion were characterized. The stability of the OB emulsions was investigated. It was found that different OB emulsions contained different types and contents of endogenous and exogenous proteins. Aggregation at low pHs (<6) and creaming at high pHs (7 and 8) both occurred for all of three OB emulsions. Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions—low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction. While a high concentration of ALG improved the OB emulsion stability through the viscosity effect at pH 7. The OB emulsions stabilized by ALG were salt-tolerant and freeze–thaw resistant.
Highlights
Oil bodies (OBs) are natural droplets with a core of liquid triacylglycerols (TAGs) surrounded by a monolayer membrane of phospholipids embedded with oil bodies (OBs) endogenous proteins [1,2]
The results showed that all three 1 wt.% of pure OB emulsions exhibited shear thinning characteristics within the shear rate range examined
Whereas, when the ALG concentration was increased to 1.2 wt.% for the sesame OB emulsion and 1.5 wt.% for the peanut and rapeseed OB emulsions, the viscosities of the OB emulsions were significantly increased, e.g., the viscosity of each was about 200–700 mPa·s between 10 s−1 and 100 s−1, which was more than 100 times higher than the pure OB emulsions
Summary
Oil bodies (OBs) are natural droplets with a core of liquid triacylglycerols (TAGs) surrounded by a monolayer membrane of phospholipids embedded with OB endogenous proteins [1,2]. These surface proteins include mainly oleosin (15~25 kDa), caleosin (27~30 kDa), and steroleosin (~40 kDa). We stabilized the soybean OB emulsion by using ALG in different environments (pH, salt, and freeze–thaw cycle), and found that ALG can be adsorbed onto the surface of OB droplets, preventing OB aggregation at low pHs [29]. This study broadened the range of applications for diverse OBs under different conditions
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