Abstract
The effect of 21-day depletion and extending the repletion period from 7 to 14 days within the constructs of a 35-day rat liver bioassay was investigated. Male weanling Sprague-Dawley rats were depleted on a low folate AIN-76A formulated basal diet for 21 days. In repletion diets, folic acid or brewer's yeast were added to provide 0.57, 1.13, and 2.27 μM/kg. The 14-day slopes calculated for folic acid and brewer's yeast were compared to 7-day slopes from four previous studies. The present slopes were significantly different in two studies for folic acid and in one study for brewer's yeast. However, the longer repletion period improved liver folate resolution in response to dietary folate levels and the efficiency of the 35-day assay protocol.
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