Abstract
In this study, a compressed oxygen gas supply was connected to a sealed aerated stirred tank reactor (COS-SSTR) bio-system, leading to a high-oxygen pressure bioreactor used to improve the bio-transformative performance in the production of 1,3-dihydroxyacetone (DHA) from glycerol using Gluconobacter oxydans NL71. A concentration of 301.2±8.2gL(-1) DHA was obtained from glycerol after 32h of fed-batch fermentation in the COS-SSTR system. The volumetric productivity for this process was 9.41±0.23gL(-1)h(-1), which is presently the highest obtained level of glycerol bioconversion into DHA. These results show that the application of this bioreactor would enable microbial production of DHA from glycerol at the industrial scale.
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