Abstract
The fruits of wild rose (Fructus Rosae) have been widely used in both traditional and officinal medicine for the long time. The pharmacological effect of the Rosehip medicine is related to the content of such biologically active compounds as: water-soluble (ascorbic acid) and fat-soluble (carotenoids) vitamins, as well as flavonoids (astragalin, isoquercitrin, tiliroside). Such medicine as Holosas, Carotolin and Rosehip syrup are obtained from the fruits of this plant. In addition, wild rose fruits are part of the various fees from which patients could prepare water infusions at home themselves.
 Regulatory documentation provides for the analysis of Rosehip raw materials three parameters: the content of ascorbic acid, the total carotenoids and flavonoids. The quantitative content of the total carotenoids in the fruits of the rosehip is regulated for the production of rosehip oil and Carotolin. However, in our opinion, the method of quantitative determination of the total carotenoids, described in the State Pharmacopoeia of the Russian Federation of the XIV edition, is too complicated and contains an unreasonably entered coefficient in the formula, leading to an overestimation of the analysis results in 10 times.
 We have developed the methodic of quantitative determination of the total carotenoids in Rosehip, that include single extraction of raw materials with hexane at room temperature and periodic mixing in the ratio of «raw material : extract» 1 : 30. Investigated solution there was obtained by diluting the hexane extract in the ratio of 2:25 and measured the optical density at wavelength 450 nm. Adjustments have been made to the formula for the calculating of the total carotenoids relating to the removal of the coefficient 10 unjustifiably entered into the pharmacopoeial methodic. It was determined that the content of the total carotenoids calculated on ß-carotene varied from 10.15 to 74.5 mg%. The metrological characteristics of the methodic indicate that the error of a single determination of the total carotenoids in the rosehip fruits with a 95% confidence level is ± 3.57%. Based on the results of the studies, a new test quality indicators “content of the total carotenoids at least 30 mg%” was substantiated instead of 300 mg%.
Highlights
Нормативная документация предусматривает анализ сырья «Шиповника плоды» по трем параметрам: содержанию аскорбиновой кислоты, суммы каротиноидов и флавоноидов
Сумму флавоноидов в пересчете на рутин определяют с использованием дифференциальной спектрофотометрии [16]
We have developed the methodic of quantitative determination of the total carotenoids in Rosehip, that include single extraction of raw materials with hexane at room temperature and periodic mixing in the ratio of «raw material : extract» 1 : 30
Summary
Для обоснования методики количественного определения суммы каротиноидов нами проведен сравнительный анализ образцов шиповника собачьего (Rosa canina L.) – Ботанический сад Самарского университета (2018 г.) и шиповника коричного (Rosa cinamommea L.) – Средне-Волжский филиал ФГБНУ ВИЛАР 2015 г. и 2018 г. Для обоснования методики количественного определения суммы каротиноидов нами проведен сравнительный анализ образцов шиповника собачьего (Rosa canina L.) – Ботанический сад Самарского университета (2018 г.) и шиповника коричного (Rosa cinamommea L.) – Средне-Волжский филиал ФГБНУ ВИЛАР 2015 г. Цельное лекарственное растительное сырье (ЛРС) следующих производителей: ООО Фирма «Здоровье» Измельченное ЛРС (фильтр-пакеты) следующих производителей: ООО Фирма «Здоровье» Определение содержания суммы каротиноидов в сырье шиповника проводили с помощью спектрофотометрии (спектрофотометр «Specord 40», Analytik Jena) с использованием фармакопейной методики (ГФ РФ XIV издания) [16], а также методики, разработанной нами в ходе настоящего исследования. Для полной характеристики сырье также анализировали на предмет соответствия по содержанию суммы флавоноидов и аскорбиновой кислоты с использованием фармакопейных методик.
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