Abstract
Sequential statistical experimental designs (Plackett–Burman, factorial, response surface and ridge analysis) were used to improve the medium for the production of Echinocandin B (ECB) by Aspergillus nidulans. Special attention is given to the ridge analysis, a search and optimization technique which can be applied to second-order models. Fifteen medium ingredients were investigated using a total of 108 shake flask fermentations over five stages of experimentation. The iterative process of thoughtful design and careful data analysis ultimately produced a simple, five component media, which increased titre by 46%.
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