Abstract
Active ingredients of medicinal plants have unique pharmacological and clinical effects. However, conventional extraction technology has many disadvantages, such as long-time and low-efficiency. XynA-assisted extraction may overcome such problems, since the plant cell wall is mainly composed of cellulose. Based on the three-dimensional protein structure, we found the C-terminal domain and N-terminal domain twisted together and resulted in more flexibility. We carried out a series of truncations, with XynA_ΔN36 getting more yields of active ingredients. As shown by HPLC analysis, the efficiencies for extraction of salvianic acid A and berberine from Salvia miltiorrhiza and Phellodendron chinense were increased by approximately 38.14% and 35.20%, respectively, compared with the conventional extraction protocol. The yields of the two compounds reached 2.84 ± 0.05 mg g−1 and 3.52 ± 0.14 mg g−1, respectively. Above all, XynA_ΔN36 can be applied to the extraction of salvianic acid A and berberine, and this study provides a novel enzyme for the extraction technology, which aids rational utilization and quality control of medicinal plants.
Highlights
Medicinal plants play important roles in the treatment of clinical diseases
Because the active ingredients mostly come from roots and stems, roots of Salvia miltiorrhiza and bark of Phellodendron chinense were selected. e incubation condition of enzyme was optimized as previously reported [20]
XynA-assisted extraction was found to significantly enhance the extraction of active ingredients in medicinal plants, and this method was successfully applied to the extraction of salvianic acid A and berberine
Summary
Medicinal plants play important roles in the treatment of clinical diseases. They may benefit patients infected by 2019-nCoV and patients with Alzheimer’s disease [1, 2]. Over the past few decades, the pharmacological effects of active ingredients from medicinal plants have attracted increasing international attention. Many research teams aimed to optimize the processes of traditional extraction and established green, economical, and low-consumption methods for rapid analyses of complex compounds in medicinal plants [5, 6]. Compared to traditional organic reagent extraction, enzyme-assisted extraction could stabilize the chemical structure of active ingredients in a wild way. Enzyme-assisted extraction could avoid the organic solvent effect towards the pharmacodynamics study
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
