Abstract
The use of T1 lipase in automatic dishwashing detergent (ADD) is well established, but efficiency in hard water is very low. A new enzymatic environmentally-friendly dishwashing was formulated to be efficient in both soft and hard water. Thermostable enzymes such as T1 lipase from Geobacillus strain T1, Rand protease from Bacillus subtilis strain Rand, and Maltogenic amylase from Geobacillus sp. SK70 were produced and evaluated for an automatic dishwashing detergent formulation. The components of the new ADD were optimized for compatibility with these three enzymes. In compatibility tests of the enzymes with different components, several criteria were considered. The enzymes were mostly stable in non-ionic surfactants, especially polyhydric alcohols, Glucopon UP 600, and in a mixture of sodium carbonate and glycine (30:70) buffer at a pH of 9.25. Sodium polyacrylate and sodium citrate were used in the ADD formulation as a dispersing agent and a builder, respectively. Dishwashing performance of the formulated ADDs was evaluated in terms of percent of soil removed using the Leenert‘s Improved Detergency Tester. The results showed that the combination of different hydrolysis enzymes could improve the washing efficiency of formulated ADD compared to the commercial ADD “Finish” at 40 and 50 C.
Highlights
Detergent is a substance containing a certain chemical that can remove dirt
The stability of T1 lipase, Rand protease and Maltogenic amylase were checked via a compatibility test
The results show that non-ionic surfactants were compatible for all three enzymes except SDS, a cationic surfactant, which destabilized all three enzymes
Summary
Detergent is a substance containing a certain chemical that can remove dirt. Detergents consist of highly-developed surfactants and water softeners. These detergents are superior to soaps because they can perform better washing in the presence of metallic ions such as Ca2+ and Mg2+. Sodium tripolyphosphate is a popular surfactant because of its high chelating property. On the other hand, improve washing via active oxygen species in redox reactions. Increasing use of these two chemicals has caused ecological issues when they enter water streams. Phosphates cause massive algal growth (eutrophication), while chlorines kill fish, disturbing the ecosystem’s equilibrium [1]
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