Abstract

Biosurfactants have many advantages outside chemical one, led for application it through different sectors. So, the present study aimed for improving the bioremediation technology of contaminated environments using biosurfactants produced by novel bacillus isolates. In this regard, novel Egyptian Bacillus thuringiensis and Bacillus toyonensis strains were obtained as most producing isolates of highly active biosurfactants. The optimized conditions for high biosurfactants yield production were established. Also, the stability of the produced biosurfactants at various conditions, pH, temperature and salinity was studied. The biosurfactant has been reported up to 120 ˚C, pH 12 and 10% NaCl. The identified biosurfactants, Decanoic acid and oleamide were applied for wastewater remediation from oil residues and pathogens contamination. The biosurfactant was had high antibacterial activity compared with references antimicrobial drugs, as well as it is enhanced bioremediation technology for petroleum oil residues contaminating sites. Thus, we can say, these biosurfactants could achieve the objectives of sustainable development.

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