Abstract
BackgroundTraditional crop breeding has made significant achievement meet food needs worldwide. However, the way has some inevitable problems including time-consuming, laborious, low predictability and reproducibility. In this study, we updated the GRAIN SIZE 3 (GS3) locus to improve the grain length of a major cultivate variety of Kongyu 131 at Heilongjiang Province, the northernmost region of China. High-resolution melting (HRM) analysis is used for single nucleotide polymorphism (SNP) genotyping.ResultsThe improved line introgressed about 117 kb segment including gs3 allele from donor GKBR by using five SNP markers designed within and without GS3 locus, and the background recovery ratio of the recurrent parent genome is about 99.55% that are detected by 219 SNP markers evenly distributed on the 12 chromosomes. The field trial indicates that grain length, 100-grain weight and total grain weight per plant of the improved line raised by 12.05%, 16.30% and 4.47%, respectively, compared with Kongyu 131.ConclusionsThis result demonstrates that update the GS3 locus is a feasible and efficient and accurate way can be applied to improve grain size of rice.
Highlights
Traditional crop breeding has made significant achievement meet food needs worldwide
To improve the grain size of the elite variety Kongyu 131, we carried out Quantitative trait loci (QTL) analysis with grain size and detected desirable allele around the GRAIN SIZE 3 (GS3) locus
QTL analysis detected a grain length related locus nearby GS3 To detect allele can increase Kongyu 131’s grain length, we measured grain length and analyzed genotype with an F2 populations constructed by Kongyu 131 and GKBR, and made QTL analysis according to Mapmaker/ QTL 1.1b (Lincoln et al 1993)
Summary
Traditional crop breeding has made significant achievement meet food needs worldwide. High-resolution melting (HRM) analysis is used for single nucleotide polymorphism (SNP) genotyping. It is a great challenge enhancing production of crops to supply the increasing demand continuously; on the one hand, the arable land is decreasing with the development of urbanization, on the other hand, the production of crop is influenced inevitably by some environment factors of global warming more and more (Takeda and Matsuoka 2008). It is an urgent challenge to supply the demand of accumulative population by increasing crop productivity. Rice is a staple crop cultivated extensively, and it supply over half diets of population all around the world. Traditional breeding almost depends on field selection by experience of breeders to improve crop variety.
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