Abstract
Malignant brain tumors are among the deadliest neoplasms with the lowest survival rates of any cancer type. In considering surgical tumor resection, suboptimal extent of resection is linked to poor clinical outcomes and lower overall survival rates. Currently available tools for intraoperative histopathological assessment require an average of 20 min processing and are of limited diagnostic quality for guiding surgeries. Consequently, there is an unaddressed need for a rapid imaging technique to guide maximal resection of brain tumors. Working towards this goal, presented here is an all optical non-contact label-free reflection mode photoacoustic remote sensing (PARS) microscope. By using a tunable excitation laser, PARS takes advantage of the endogenous optical absorption peaks of DNA and cytoplasm to achieve virtual contrast analogous to standard hematoxylin and eosin (H&E) staining. In conjunction, a fast 266 nm excitation is used to generate large grossing scans and rapidly assess small fields in real-time with hematoxylin-like contrast. Images obtained using this technique show comparable quality and contrast to the current standard for histopathological assessment of brain tissues. Using the proposed method, rapid, high-throughput, histological-like imaging was achieved in unstained brain tissues, indicating PARS’ utility for intraoperative guidance to improve extent of surgical resection.
Highlights
Malignant brain tumors are among the deadliest neoplasms with the lowest survival rates of any cancer type
In this work we present a multiwavelength rapid acquisition photoacoustic remote sensing (PARS) system designed to directly emulate the contrast of histopathological staining in human brain tissues, the widely used hematoxylin and eosin (H&E) staining
In order to mimic the contrast of H&E and generate false-color PARS images, a 1 kHz tunable excitation laser was used to target the same biomolecules highlighted in H&E staining
Summary
Malignant brain tumors are among the deadliest neoplasms with the lowest survival rates of any cancer type. A fast 266 nm excitation is used to generate large grossing scans and rapidly assess small fields in real-time with hematoxylin-like contrast Images obtained using this technique show comparable quality and contrast to the current standard for histopathological assessment of brain tissues. Rapid, high-throughput, histological-like imaging was achieved in unstained brain tissues, indicating PARS’ utility for intraoperative guidance to improve extent of surgical resection. Injury potentially driving excess resection and unnecessary neurological damage to patients[11] This method is slow, and like standard MRI lacks microscopic precision, contrast and tissue specificity[11,12]. There remains an urgent need for a rapid interoperative in-situ histopathological imaging technique to aid in safely and maximally resecting brain tumors
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