Abstract
Brazilin was one of phytoconstituent from sappanwood that widely used as textile-colouring agent and found in traditional concoction for therapeutic purposes. Due to obtain its maximum level in sappanwood (Caesalpinia sappan L.) extract, the extraction method of brazilin has been developed. Enzyme-assisted extraction (EAE) is one of green chemistry methods to achieve that outcome. This study aims to enhance brazilin level by optimize the EAE condition with fungi cellulase. The cellulase are produced by monoculture Aspergillus niger UICC371 in carboxymethyl cellulose submerged fermentation’s media. Sappanwood extracted with fungi cellulase through variation conditions: enzyme concentrations (2.0; 4,0; 6.0%); temperature (45, 50, 55℃); and time (1, 2, 3 hrs). The optimization are provided by response surface method-BoxBehnken design and brazilin level was carried out through High Performance Liquid Chromatography (HPLC) with asetonitril : 0,3% acetic acid in water (14.5 : 85.5) as eluents. The study showed that cellulase from monoculture of Aspergillus niger UICC371 showed optimum condition of cellulase-EAE method at 6.0% concentration enzyme at 50℃ for 3 hours extraction time which provide an increase in brazilin level to 5.014% compare to reflux method. Key Words: Caesalpinia sappan L., Cellulase, Enzyme assisted extraction, Fungi, Response surface method.
Highlights
Sappanwood (Caesalpinia sappan L.) plants (Figure 1) belongs to Fabaceae family which the heartwood commonly used as textile and food colouring agents.[6]
This study aims to enhance brazilin level by optimize the enzyme-assisted extraction (EAE) condition with fungi cellulase
The study showed that cellulase from monoculture of Aspergillus niger UICC371 showed optimum condition of cellulase-EAE method at 6.0% concentration enzyme at 50°C for 3 hours extraction time which provide an increase in brazilin level to 5.014% compare to reflux method
Summary
Sappanwood (Caesalpinia sappan L.) plants (Figure 1) belongs to Fabaceae family which the heartwood commonly used as textile and food colouring agents.[6]. The conventional method to extract brazilin from sappanwood had been observed with some eluents (ethanol and methanol). Other extraction method, Green-Chemistry method, is more promising recently. That method is widely developed and being an alternative to conventional that used organic eluents. 11 That method can improve an active phytoconstituents of natural products-extract than conventional methods. 9,14 Microwave assisted extraction (MAE), ionic liquid, and its combination methods were two of them that had observed previously. 9,14 that methods yielded lack of brazilin (0.1—0.9%) 9 so that need to be improved by other promising method, which used enzyme or by enzyme-assisted extraction (EAE) method That method is widely developed and being an alternative to conventional that used organic eluents. 11 That method can improve an active phytoconstituents of natural products-extract than conventional methods. 9,14 Microwave assisted extraction (MAE), ionic liquid, and its combination methods were two of them that had observed previously. 9,14 that methods yielded lack of brazilin (0.1—0.9%) 9 so that need to be improved by other promising method, which used enzyme or by enzyme-assisted extraction (EAE) method
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
