Abstract
The effects of electron beam irradiation pretreatment on the structural and compositional features and on the enzymatic saccharification of hybrid poplar were investigated. The carbohydrate compositional analyses with 1H-NMR spectroscopic method were applied in this study to investigate the change in the polysaccharides of hybrid poplar quantitatively. As the irradiation dose increased, the amount of extract produced in mild alkaline extraction was increased. The composition of the residual biomass after mild alkaline extraction was also changed depending on the electron beam irradiation dose. Especially for xylose, the peak intensity in NMR spectra was decreased with increasing electron irradiation dose, which demonstrated the significant decomposition of xylan caused by the electron beam irradiation. The changes induced by electron beam irradiation resulted in a better response to enzymatic hydrolysis with commercial cellulases (Celluclast 1.5 L and Novozym 342). More improvement in enzymatic hydrolysis by the irradiation was found in the hydrolysis of the xylan than that of the cellulose in hybrid poplar.
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