Abstract
AbstractPseudomonas fluorescensP5 was isolated in Yunnan Province and proved to be an effective biocontrol agent of various soil-borne plant diseases. The aim of the study was to enhance the biocontrol effect of this strain by introducing a chitinase gene. A 6.5 kb DNA fragment containing the chitinase genechiBwas inserted into pDSK519 vector to construct a new plasmid, pDSK51965. This plasmid was introduced intoP. fluorescensP5 and resulted in a new strain named P5-1. Restriction enzyme digestion and chitin plate culture confirmed that P5-1 contained a functional chitinase gene. Compared with wild-type P5 in pot experiments, P5-1 had an increased effect against rice sheath blight and cotton damping-off caused byRhizoctonia solani. It also increased the effect on suppression of wheat take-all caused byGaeumannomyces graminisvar.tritici.
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