Improving Biochar Suitability for Arid-Land Use: Impact of Elemental Sulfur and Compost on Acidification and Biological Activation

Abstract

This study aimed to enhance the quality of biochar for applications in arid lands by employing elemental sulfur (S, 0.013%) as an acidifying agent and compost (10%) as a biological activator. The addition of elemental sulfur significantly reduced the biochar pH, with the most substantial decrease from 8.1 to 7.2 observed when co-amended with vermicompost was used. Elemental sulfur markedly increased the water-soluble concentrations of calcium (Ca) by 147% and 105%, as well as magnesium (Mg) by 929% and 447% in compost and vermicompost, respectively. This suggests a decline in biochar basicity due to acid mineral dissolution and desorption. Sulfate (SO42-) levels showed the greatest increase when compost was co-applied with sulfur, indicating more efficient oxidation in this treatment. FT-IR analysis revealed increased carbonyl groups (C=O) and decreased alkyne (C≡C) groups in compost and vermicompost-treated samples, while sulfur treatment resulted in the decrease of hydroxyl groups, but only in the presence of vermicompost. SEM-EDX analysis demonstrated changes in the elemental composition and microscale pore structure of biochar samples after incubation with sulfur. Sulfur amendments stimulated substrate-induced respiration, particularly in biochar amended with sulfur (BS, 0.011 ug CO2- C/g/h) and biochar with vermicompost (BV, 0.18 ug CO2- C/g/h) treatments. Microbial diversity (Shannon H) significantly increased in compost treatments with sulfur amendment from 3.08 to 4.52, while it decreased in vermicompost from 4.25 to 4.02. Vermicompost treatments (BV, BVS) exhibited higher microbial evenness (0.27 and 0.28) and equitability (0.67) diversity indices. The bacterial community structure was significantly influenced by all treatments, with sulfur reducing the abundance of Proteobacteria by 30% in the presence of compost and 8% with vermicompost, while increasing the abundance of Actinobacteria by 18% and 4% for compost and vermicompost treatments, respectively.Multivariate principal component analysis (PCA) indicated that soluble sulfate was associated with specific sulfur-oxidizing bacterial clusters, which were differentially expressed under different compost treatments. Integrating biochar with sulfur and compost emerges as a promising sustainable technology for managing alkalinity, enhancing soil fertility, and improving agricultural productivity in arid regions.