Improving antibacterial activity and viability of Lactobacillus plantarum AKK30 as feed additive by addition of different oligosaccharides

Abstract

Combination of lactic acid bacteria as probiotic with oligosaccharides substances has potentially improved nutrient utilization and poultry performance. A study was conducted to evaluate-the addition of oligosaccharides inulin or mannan oligosaccharides (MOS) with different level of oligosaccharides into growth medium on antibacterial activity and viability of Lactobacillus plantarum AKK30 in vitro. The experiment was arranged on the factorial design using two factors, the A factors were oligosaccharides types (inulin or MOS), and the B factors were increased level of oligosaccharides (0, 0.5, 1, 1.5 and 2%). Each treatment was conducted in 3 replications. Data were analyzed with analysis of variance (ANOVA) among the treatments that show significant differences (p<0.05) followed by Duncan post hoc test. The results showed that the combination of L. plantarum and oligosaccharides (P<0.05) influenced L. plantarum growth according to the value of OD (0D-590nm). However, a combination of L. plantarum and oligosaccharides was not significant (p>0.05) for antibacterial activity. Addition of mannan oligosaccharides (MOS) 0.5 - 2% could increase growth of L. plantarum growth with 0.5% MOS as optimum level.