Abstract

The resistant structure and high carbon/nitrogen ratio (C/N) of cellulosic substrate are the barriers during their anaerobic digestion (AD). Solid-state urea pretreatment was developed in this study to pretreat corn straw and adjust C/N ratio simultaneously for the downstream AD of corn straw. Results showed solid-state urea pretreatment was efficient in lignin removal and achieved the highest lignin reduction of 7.06% with C/N ratio = 15 during pretreatment. Scanning electron microscopy demonstrated the destruction of the dense structure by pretreatment, which benefited to the hydrolysis of corn straw. The cumulative methane yields of the pretreated corn straw ranged from 234.07 to 250.03 mL/g VS, which were obviously higher than that of the untreated corn straw. The maximum methane yield of 250.03 mL/g VS was achieved with C/N = 15 during pretreatment, which was 23.91% higher than that of the untreated group. In addition, AD digestates from the pretreated groups had 9.62% higher nutrients than that from the untreated group. The solid-state urea pretreatment can destroy the dense structure of corn straw and regulate the C/N ratio during AD, thus benefit the methane production and fertilizer use of the digestate, which is a potential choice during the AD of cellulosic substrate.

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