Improvement on the extraction efficiency of low density lipoprotein in an ionic liquid microemulsion

Abstract

A novel microemulsion is developed at room temperature with 30 µL of sodium alginate sulfate (SAS, 0.02 mol/L), 0.005 g bis (2-ethylhexyl) succinate sulfonate (AOT) and 270 µL of 1-butyl-3-methylimidazolium hexafluorophosphate (BmimPF6) ionic liquid as aqueous phase, surfactant and IL phase, respectively. The SAS/AOT/BmimPF6 microemulsion significantly improves the extraction efficiency for low density lipoprotein (LDL). 96% LDL in a 300 µL of PBS is selectively extracted into a same volume of microemulsion, with respect to those of 67%, 76% and 85% by BmimPF6, H2O/AOT/BmimPF6 microemulsion and sodium alginate (SA)/AOT/BmimPF6 microemulsion. LDL in the SAS/AOT/BmimPF6 microemulsion is distributed both in BmimPF6 via hydrophobic interaction and in the “pools” of the microemulsion via electrostatic interaction with AOT and specific interaction between LDL with SAS. 83% of LDL in the microemulsion can be readily back extracted into an aqueous phase with 0.8% (m/v) of sodium dodecyl sulfate (SDS) as stripping reagent. For practical applications, LDL in human serum is selectively extracted with the microemulsion, as demonstrated by enzyme linked immunosorbent assay (ELISA) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).