Abstract

In our previous research, the immobilization of formaldehyde dehydrogenase (FDH) onto mesoporous silica (MPS: pore size = 12.3 nm) was investigated. However, this method could not obtain the thermal stability of the enzyme. To solve this problem, FDH was immobilized on mesoporous zirconia material (MPZ) with a lower thermal conductivity. MPZ was synthesized using [poly(ethylene glycol)–poly(propylene glycol)–poly(ethylene glycol) (Pluronic P123, EO20PO70EO20)], zirconium(IV) n-propoxide (ca. 75% in 1-propanol), acetylacetone, 1, 3, 5-trimethylbenzene, and ethanol. The material retained high surface area (113.6 m2/g) and pore volume (0.27 cm3/g). It was characterized by Brunauer–Emmett–Teller surface area, Barrett–Joyner–Halenda pore size distribution, X-ray diffraction, field-emission scanning electron microscopy, transmission electron microscopy, and energy-dispersive X-ray spectroscopy.

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