IMPROVEMENT OF THE THREE-DIMENSIONAL VISUALIZATION METHODS FOR DETERMINATION OF MORPHOLOGICAL FEATURES OF PHASE MICROOBJECTS

Abstract

The use of methods of physical and mathematical three-dimensional modelling of phase microobjects, in particular blood erythrocytes, was proposed in order to determine morphological changes. The method of digital signal processing based on double Fourier transform, with the imposition of a band pass filter, the method of restoring the three-dimensional shape of objects from two-dimensional images and the method of spectral analysis were considered in detail improved performance. On the basis of dimensional and aberration calculations, the optimal elements in terms of optical parameters were selected and an experimental stand was assembled. Interference digital images of normal human erythrocytes and altered under the action of osmotic pressure, images of peripheral blood cells for illumination by three types of coherent radiation were obtained. To implement three-dimensional modelling developed a program in Python using Numpy and Open CV libraries and a program that allows to build and visualize three-dimensional models of two-dimensional images, running Windows, written in C ++. A DirectX library and a graphics accelerator are used to display 3D models. The method of visualization of three-dimensional models on two-dimensional images makes it possible to change the scale of the surface and the type of projection used, to work in real time in interactive mode. The method of spectral analysis with processing of images of erythrocytes for enlightenment obtained in three frequency bands of coherent light, after further correlation of the obtained results will significantly improve the accuracy of determining its morphological parameters. The proposed improved methods allow using them to determine the state of the plasma membranes of erythrocytes in order to assess possible pathologies, which will increase the effectiveness of laboratory diagnostic methods.