Abstract
The sandwich ELISA for the determination of basement membrane collagen complex (BCC), which is composed of type IV collagen and basement membrane-associated collagen (BAC), in sera of patients with chronic liver diseases was improved by using the chaotropic ion, NaSCN. NaSCN activated the immunoreactivity of endogenous BCC in sera in a concentration-dependent manner and showed the maximum effect at 400 mM. Intra- and inter-assay coefficients of variation were below 6.9% and below 11.9%, respectively. Recovery of standard BCC added to a normal or a pathological serum was 102.4 to 108.0%. The BCC levels in sera of patients with chronic hepatitis and liver cirrhosis showed significantly higher values than those in serum of healthy volunteers, which was consistent with the immunohistochemical analysis in which the staining of BAC of the liver cirrhotic tissue was much increased as compared with that of normal liver tissue.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
