Improvement of the in vitro recordings in cortical slices by using customized flexible neuroprobes

Abstract

Event Abstract Back to Event Improvement of the in vitro recordings in cortical slices by using customized flexible neuroprobes Xavi Illa1, 2*, Beatriz Rebollo3, Gemma Gabriel1, 2, Maria V. Sanchez-Vives3, 4 and Rosa Villa1, 2 1 CIBER-BBN, Spain 2 IMB-CNM, CSIC, Spain 3 IDIBAPS, Spain 4 ICREA, Spain We have explored the feasibility of improving the quality of multiple recordings from spontaneously oscillating cortical slices. With that purpose we have taken advantage of the advances performed in the development of flexible neuproprobes, either fabricated on polyimide, SU-8, PDMS or parylene (Hassler et al. 2011). With these materials the contact between the electrodes and the tissue is enhanced with respect to the neuroprobes fabricated on rigid substrates, such as silicon or pyrex. However, we had to find a compromise between the necessity to achieve a good contact between the electrode and the slice and the need to allow the flow of oxygenated solution to the slice to maintain its healthy state when using flexible neuroprobes. To overcome this limitation, we have designed, fabricated and characterized a 16-electrode flexible neuroprobe that allocates an array of holes in its sensing area. This neuroprobe has been developed using SU-8 negative photoresist as a substrate material in the clean room facilities of the IMB-CNM. In particular, the neuroprobes have been fabricated following the process described in a previous article from the authors (Guimerà et al. 2013); using silicon wafers with an aluminum sacrificial layer as a support for the fabrication process. Then, the neuroprobes were released from the wafer by an anodic dissolution of the aluminum layer (Metz et al. 2005). In order to validate the usability of the fabricated device, the neuroprobes were used to record spontaneous slow oscillations to study the wave propagation along the cortical networks while manipulating them by means of pharmacological drugs or electric fields. We conclude that the perforated devices provide substantial improvement in the adherence of the electrodes to the tissue, on the mechanical stability of the recordings, and in the healthiness of the slices. Acknowledgements This work has been supported by the Spanish Ministry of Economy and Competitiveness, BFU2011-27094 (MVSV) and SAF2012-40227- C02-02 (RV), the European Regional Development Fund (RV), EU project CORTICONIC (Contract number 600806, MVSV) and the Graphene Flagship Initiative of the European Commision via the NEUROGRAPHENE project (Contract 604391, MVSV and RV). References Hassler, C., Boretius, T., Stieglitz, T. (2011). Polymers for neural implants. J. Polym. Sci. Pol. Phys. 49, 18-33 Guimerà, A., Illa, X., Traver, E., Plata-Cordero, M., Yeste, J., Herrero, C., Lagunas, L., Maldonado, M. J., Villa, R. (2013). Flexible probe for in vivo quantification of corneal epithelium permeability through non-invasive tetrapolar impedance measurements. Biomed. Microdevices 15, 849-858 Metz, S., Bertsch, A., Renaud, P. (2005). Partial release and detachment of microfabricated metal and polymer structures by anodic metal dissolution. J. Microelectromech. Syst. 14, 383-391 Keywords: SU-8, Neuroprobe, cortical network, microfabrication, spontaneous activity Conference: B·DEBATE | A Dialogue with the Cerebral Cortex: Cortical Function and Interfacing (Workshop), Barcelona, Spain, 29 Apr - 30 Apr, 2015. Presentation Type: Poster Presentation Topic: SESSION 3: Interfacing with the Cerebral Cortex: New Technologies Citation: Illa X, Rebollo B, Gabriel G, Sanchez-Vives MV and Villa R (2015). Improvement of the in vitro recordings in cortical slices by using customized flexible neuroprobes. Front. Syst. Neurosci. Conference Abstract: B·DEBATE | A Dialogue with the Cerebral Cortex: Cortical Function and Interfacing (Workshop). doi: 10.3389/conf.fnsys.2015.06.00001 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 20 Apr 2015; Published Online: 06 May 2015. * Correspondence: Dr. Xavi Illa, CIBER-BBN, Barcelona, Spain, xavier.illa@imb-cnm.csic.es Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Xavi Illa Beatriz Rebollo Gemma Gabriel Maria V Sanchez-Vives Rosa Villa Google Xavi Illa Beatriz Rebollo Gemma Gabriel Maria V Sanchez-Vives Rosa Villa Google Scholar Xavi Illa Beatriz Rebollo Gemma Gabriel Maria V Sanchez-Vives Rosa Villa PubMed Xavi Illa Beatriz Rebollo Gemma Gabriel Maria V Sanchez-Vives Rosa Villa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.