Abstract
Ascorbic acid (AA) is an important compound of the human diet, in the metabolism acting mainly as exogenous antioxidant. A method for electrochemical detection of Ascorbic acid (AA) with a GCE was developed using a cationic surfactant cetylpyridinium chloride (CPC). The presence of CPC influences the electrochemical behaviour of AA, reduces the applied potential to measure AA oxidation followed by an increase in the peak current. In the optimized conditions the AA determination was possible in the linear range of 5.0 x 10-7 mol L-1 - 4.3 x 10-4 mol L-1 with a detection limit (S/N = 3) of 2.0 x 10-7 mol L-1. The applicability of the method is in the sensitivity and selectivity of the GCE for the AA detection as well as the simplicity of the demonstrated method using an electrode without modifying and without pre-treatment of samples. Recovery tests presented values of 98 - 103% suggesting the method has no significant interference and making possible to be successfully used for AA determination in complex samples.
Highlights
Ascorbic acid (AA) ( -lactone) is a water-soluble compound cited as antiscorbutic and antioxidant playing a key role in the immune response
Voltammetric curves of AA recorded in the absence of cetylpyridinium chloride (CPC) (Figure 1b) showed an irreversible cyclic voltammogram with the AA oxidation peak around +0.23 V
The observed changes in the redox parameters of AA can be attributed to a surface effect
Summary
Ascorbic acid (AA) ( -lactone) is a water-soluble compound cited as antiscorbutic and antioxidant playing a key role in the immune response. This dietary component is well-known as an effective chainbreaking antioxidant employed as additive in emulsions to prevent lipid oxidation in pharmaceutical preparations and food. The oxidation of this compound to L-dehydroascorbic acid has received a great attention because of the important role that the redox chemistry of AA plays in human nutrition. The acid character and reduction action are attributed to its enodiol group (-COH=COH-) in the structure (LIU; ANZAI, 2004)
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