Improvement of the ability of bone repair by reducing the aging degree of mesenchymal stem cell population by Senolytic drugs

Jia Liu,Lian He,Peng Zhou,Danna Chen,T Zhu,M Anpo,A Sharifi

doi:10.1051/e3sconf/202127103015

Abstract

In order to study the effects of Senolytic drugs dasatinib and quercetin on the aging of mesenchymal stem cells (MSCs) and explore the application of young MSCs in repairing bone defects, the cultured and identified MSCs were treated with dasatinib and quercetin, counting the number of passage times, and the senescence of cells was evaluated by high-content screening microscopy. The 4th-passage MSCs rejuvenated with Senolytic drugs were transplanted into bone defect model rabbits, and X-ray examination was performed 12 weeks after surgery. The results showed that Senolytic drugs reduced the senility of MSCs and improved the ability of bone repair.

Highlights

Mesenchymal stem cells (MSCs) are stem cells with multidirectional differentiation potential and are needed in large quantities in regenerative medicine [1]
After the MSCs were treated with Senolytic drugs, in order to determine the osteogenic ability of the cells in vivo, the cells were transplanted into the bone defect model rabbits, and the ability of MSCs to repair bone defects was analyzed by X-ray
MSCs cannot proliferate and differentiate indefinitely, and signs of aging will appear after a certain number of generations of replication [4], namely replicative aging

Summary

Introduction

Mesenchymal stem cells (MSCs) are stem cells with multidirectional differentiation potential and are needed in large quantities in regenerative medicine [1]. MSCs amplification will cause senescence, reduce differentiation ability, and limit its clinical application value. This study aimed to reduce the aging of MSCs and improve the ability of osteogenic differentiation. The effect of Senolytic drugs on the senile degree of MSCs was evaluated by counting of cell passage times and highcontent screening microscopy. After the MSCs were treated with Senolytic drugs, in order to determine the osteogenic ability of the cells in vivo, the cells were transplanted into the bone defect model rabbits, and the ability of MSCs to repair bone defects was analyzed by X-ray

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Results

Conclusion