Abstract
The present study was aimed to develop mutant sugarcane (Saccharum officinarum L. var. Isd. 39) using gamma radiation followed by regeneration of those mutants through in vitro technique. Five different doses of gamma radiation (10, 20, 30, 40 and 50 Gy) were applied to the sugarcane setts and among these doses 10 Gy irradiated mutant sugarcane showed the best agronomical traits. In addition, 10 Gy irradiated mutant sugarcane also contained higher sugar concentrations (14.25%) compared to control (14.23%). On the other hand, shoot tip and young folded leaf segments from the 10 Gy irradiated plants (M1 generation) were used as explants in in vitro culture. It was evident from the experiment that direct shoot regeneration was better than indirect regeneration. Young leaf segments produced the highest percentage (84%) of shoots when cultured on MS+1.0 mg/l Kin + 0.2 mg/l NAA. The mean number of shoots was recorded as 35 ± 1.12 in the same medium. Moreover, addition of 4% sucrose and 10% coconut water (CW) in the same medium increased the number of shoot up to 45 per culture. Best rooting was obtained from shoots cultured on half-strength of MS fortified with 1.0mg/l NAA and 0.5mg/l IBA. Well-rooted plantlets were transferred to poly bag containing soil and compost (2:1) for hardening. During hardening 70% plantlets survived, which were subsequently transferred to the experimental field.Jahangirnagar University J. Biol. Sci. 5(1): 1-9, 2016 (June)
Highlights
Sugarcane is a tall and relatively strong class of perennial grasses that are known to have high sugar content
It is one of the most important industrial crops in both the tropical and subtropical regions of the world and is the principal raw material for the sugar industry (Khan et al, 2004).The use of gamma radiation to induce mutation is a method that has been applied in plant breeding to increase genetic variations (Brunner, 1995)
E-mail: shamima.akter221@gmail.com tissue culture as a tool of advanced plant breeding when coupled with mutation breeding would become an important and valuable tool in the hands of plant breeders to create genetic variability for the selection of new genotypes with improved agronomic characteristics
Summary
Sugarcane is a tall and relatively strong class of perennial grasses that are known to have high sugar content. Isd. 39) by gamma irradiation and to establish a protocol for large scale plantlets production of that mutant through in vitro culture. Shoot tips and young folded leaves of M1 progeny (selected elite mother plant) were collected from the experimental field and were used as explants.
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