Improvement of sperm traits related to the high level of extra-pair fertilization in tree sparrow population under long-term environmental heavy metal pollution

Abstract

Environmental stress can affect sperm traits whose changes have been reported to be associated with extra-pair fertilization (EPF) level in natural animal populations. However, little is known regarding how exposure to environmental heavy metals influences sperm traits and EPF level in free-living bird populations. In a previous study, we found that a tree sparrow (Passer montanus) population that has been exposed to heavy metal pollution over 60 years (Baiyin, BY) exhibits increased sperm quality compared with a population from a relatively unpolluted area (Liujiaxia, LJX). The high sperm quality could be related to extra-pair mating rates. Therefore, the present study investigated EPF level (the ratio of extra-pair offspring) in tree sparrow populations from BY and LJX, and analyzed the relationship between sperm traits (morphology, velocity and quantity) and EPF success. EPF success of tree sparrows was significantly correlated with their sperm velocity (p = 0.048) and total sperm length (p = 0.045), indicating that these sperm traits were important for EPF success. Tree sparrows from the BY population produced longer sperm with lower head/flagellum ratio and faster swimming sperm and showed a significantly higher EPF level than conspecifics from LJX. Thus, adaptive variation of sperm characteristics was related to the high EPF level in tree sparrows under long-term environmental heavy metal pollution. The findings are of scientific significance for exploring the evolution of mating tactics in wild bird populations under environmental stress.