Improvement of shelf life of soymilk using immobilized protease of Oerskovia xanthineolytica NCIM 2839.

A K Sahoo,P B Dandge,V S Gaikwad,R C Ranveer,S R Waghmare

doi:10.1007/s13205-016-0479-6

A K Sahoo, P B Dandge + Show 3 more

Open Access

https://doi.org/10.1007/s13205-016-0479-6

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Journal: 3 Biotech	Publication Date: Aug 8, 2016
Citations: 2	License type: CC BY 4.0

Affiliation: Shivaji University

Abstract

Protease enzyme has lot of commercial applications, so the cost-effective production of protease using sunflower oil seed waste was carried out from Oerskovia xanthineolyitca NCIM 2839. The maximum protease production was after 24 h of incubation with 2.5 % oil seed waste concentration. O. xanthineolytica was found to produce two proteases—P1 and P2. The proteases were purified using 60 % cold acetone precipitation and DEAE-cellulose ion exchange chromatography. SDS-PAGE revealed molecular weight of P1 and P2 was 36 and 24 kDa, respectively. P1 and P2 were optimally active at pH 7.0 and pH 7.5 at temperature 35 and 40 °C, respectively. Analysis of hydrolyzed product of P1 and P2 by HPLC reveals that the P1 has endoprotease and P2 has exoprotease activity. The treated soy milk with immobilized proteases showed increased shelf life and removal of off flavor.

Highlights

Proteolytic enzymes constitute one of the most important groups of commercial enzymes
Microbial proteases play an important role in biotechnological processes and they account for approximately 59 % of the total enzymes used (Spinosa et al 2000)
Oerskovia xanthineolytica NCIM 2839 was obtained from the National Collection of Industrial Microorganisms (NCIM), Pune, India

Summary

Introduction

Proteolytic enzymes constitute one of the most important groups of commercial enzymes These enzymes have ample utilization in industrial processes, such as the detergent industry, a major consumer of proteases, as well as food and leather industries (Kumar and Takagi 1999; Gupta et al 2002). Proteases are ubiquitous enzymes occurring in wide diversity of species including plants, animals and microorganisms. The vast range led to numerous attempts to exploit their biotechnological applications and established proteases as one of the major groups of industrial enzymes (Rao et al 1998). Microbial proteases play an important role in biotechnological processes and they account for approximately 59 % of the total enzymes used (Spinosa et al 2000). Proteases are produced by wide range of microorganisms including bacteria, molds, and yeasts.

Materials and methods

Results and discussion

Conclusion

Compliance with ethical standards