Abstract
Complex extracts of the plants Azulan and Hemorigen were separated by zonal micropreparative thin-layer chromatography in sandwich chambers of the ES and DS type which permitted zonal application of large volumes of sample, without auxiliary equipment. Application from the edge of the layer, in the frontal chromatography mode, markedly improved the separation efficiency and capacity owing to displacement effects which narrow the initially broad zones. Further improvement of separation efficiency and purity of fractions, revealed by densitometry, was observed using stepwise gradient elution. This was confirmed by extraction of some of the separated fractions from the layer and rechromatography; the composition of these fractions were generally simpler than for the corresponding isocratic chromatograms.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
