Improvement of Semen Quality in Rams by Adding Some Biostimulants During Cooling

Abstract

This study was carried-out to throw the light on the effect of some biostimulants as L-carnitine; 0.1mg/ml , Omega 3; 10 ng/ml and moringa olifera; 100 μg/ml on sperm progressive motility and livability percentages, abnormalities, acrosomal integrity of rams semen cooled in refrigerator (+ 5) during different periods (0,24,48,72,96 hrs) in different extender [Egg yolk citrate extender 10% egg yolk (EG) (1st dil) , tris based extender 2.5% EG (2nd dil), tris based extender 10% EG (3rd dil) ]. The results indicated that, the best treatment group that improved the sperm abnormalities and acrosomal integrity % observed in L-carnitine treated group followed Omega3-treated group and the least treatment observed in Moringa olifera treated group. The best treatment improved the progressive sperm motility and livability percentage of sperm cells observed in the groups treated with Omega-3, followed moringa olifera and the least observed in L-carnitine treated group, also the best diluent is tris based extender 10% egg yolk (3rd) the results also cleared that, by increasing the time of cooling there is a decrease of sperm quality and its ability to conception. It could be concluded that, the biostimulants used in this study specially Omega-3, and moringa olifera in tris based extender 10% egg yolk (3rd) improved semen quality of rams preserved at refrigerator for 4 days specially