Abstract
ABSTRACT Selenium-enriched yeast can transform toxic inorganic selenium into absorbable organic selenium, which is of great significance for human health and pharmaceutical industry. A yeast Rhodotorula glutinis X-20 we obtained before has good selenium-enriched ability, but its selenium content is still low for industrial application. In this study, strategies of process optimization and transport regulation of selenium were thus employed to further improve the cell growth and selenium enrichment. Through engineering phosphate transporters from Saccharomyces cerevisiae into R. glutinis X-20, the selenium content was increased by 21.1%. Through using mixed carbon culture (20 g L−1, glycerol: glucose 3:7), both biomass and selenium content were finally increased to 5.3 g L−1 and 5349.6 µg g−1 (cell dry weight, DWC), which were 1.14 folds and 6.77 folds compared to their original values, respectively. Our results indicate that high selenium-enrichment ability and biomass production can be achieved through combining process optimization and regulation of selenium transport.
Highlights
Selenium (Se) is a trace element essential for human health [1–3]
We have identified a strain of yeast, Rhodotorula glutinis X-20, which shows good selenium enrichment and growth performance [18]
Because selenite absorption depends on transporters of phosphate or monocarboxylate [7], phosphorus stimulation and a transformed phosphate transport system were employed to improve selenium enrichment
Summary
Selenium (Se) is a trace element essential for human health [1–3]. The World Health Organization (WHO) has reported that selenium deficiency is one of the most universal nutritional deficiencies in the world [4]. Selenium deficiency affects approximately 0.5–1 billion people around the world and is prone to causing health disorders such as cancer, Keshan disease, Kashin-Beck disease, muscular syndrome, and even death [3–5]. The average dietary intake of selenium by Chinese adults is only 26–32 μg per day, which is much less than the WHO-recommended intake of 40–200 μg per day [2]. Developing selenium-enriched products is of great significance to improve health standards, especially of communities whose nutrition is absent or deficient in organic selenium
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