Abstract
4-(4-Ferrocenephenyliminomethyl)phenol (FP1) was used to modify screen-printed carbon electrodes (CEs). It was shown that FP1 efficiently mediates the electron flow from pyrroloquinoline quinone (PQQ)-dependent alcohol (ADH) or glucose dehydrogenase (GDH) to the electrode surface. This CE modification increased the stability of immobilized enzymes as well. Moreover, FP1 sufficiently lowered the oxidation/reduction potential of H2O2. The FP1-modified CEs were integrated into the flow-through amperometric cell and applied for determination of H2O2, ethanol and glucose in beverages. Good correlations (r=0.9778 and r=0.9204 for glucose and ethanol, respectively) between results obtained by these biosensors and by refractometric or hydrometric methods were observed.
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