Improvement of non-specific immunity, intestinal health and microbiota of crucian carp (Carassius auratus) juvenile with dietary supplementation of Bacillus coagulans BC1

Abstract

Establishing beneficial intestinal microbiota using autochthonous probiotics is a promising approach for disease control and growth regulation during fish rearing. Therefore, it is crucial to identify indigenous probiotics with high application potential in aquaculture. In this study, we screened Bacillus coagulans BC1, a strain with strong application potential. After a comprehensive safety assessment, diets containing varying doses of BC1 were formulated and administered to crucian carp. Subsequently, a feeding experiment was conducted to evaluate non-specific immune response, intestinal structure, microbiota, and challenge protection rate. The results demonstrate that oral supplementation of BC1 can lead to tissue-dependent upregulation of various serum immune enzymes and IgM levels, while significantly enhancing the expression of IL-1β, IL-10, TNF-α, and TGF-β in various organs of crucian carp (p < 0.05). The BC1 diet effectively promoted intestinal morphology in crucian carp and improved resistance to Aeromonas veronii TH0426 infection. Intestinal microbiota analysis revealed that the oral addition of BC1 significantly changed the intestinal microbial community structure of crucian carp but did not clearly affect intestinal microbial diversity. Oral administration of BC1 significantly improved the relative abundance of Cetobacterium in the intestines of crucian carp; reduced the abundance of opportunistic pathogens such as Bacteroides, Vibrio and Aeromonas and effectively regulating the nutrient metabolism in intestinal microbiota. These results indicate that BC1 could regulate immune function, effectively improving the intestinal morphology and intestinal flora structure and function of crucian carp, and therefore is a potential candidate strain for aquatic feed additives.