Improvement of myocardial injury and gut microbiota disturbance in type 2 diabetic mice by inulin with various degrees of polymerization

Abstract

This study is to explore the effects of low-performance inulin (SI, DP < 10) and high-performance inulin (HI, DP > 10) on myocardial injury in type 2 diabetic mice fed a high-fat diet (HFD) and its underlying mechanism. Six weeks of supplementation of type 2 diabetic mice with HI significantly improved blood glucose, insulin, and triglyceride levels in comparison with SI (p < 0.05). HI had more advantageous effects in ameliorating myocardial injury by suppressing TNF-α, IL-1β, and CK-MB and promoting SDF-1β than SI. Compared to SI, HI more significantly reduced the abundance of Firmicutes from 60.5% to 36.3% in type 2 diabetic mice fed a HFD and increased the abundance of Muribaculum, Blautia and Ruminococcaceae UCG−011 at the genus level (p < 0.05). Metabolomics revealed that both SI and HI supplementation regulated tryptophan metabolism, bile acid metabolism, sphingolipid metabolism, and arachidonic acid metabolism, whereas HI supplementation increased prostaglandin h2 compared to SI. In summary, HI exhibited superior positive regulation of myocardial injury and gut microbiota dysfunction compared to SI.