Abstract
To investigate the role of maltose stearic acid ester (MSAE) for improving raw-starch-digesting glucoamylase (RSDG) production by Aspergillus niger F-01 and its regulatory mechanism. RSDG activity was enhanced 8.3-fold by adding 5g MSAE to 1l basal medium at the beginning of the culture. RSDG biosynthesis nearly ceased by adding 100μg actinomycin D/ml or 50μg cycloheximide/ml in a 48h culture in the basal medium. RSDG biosynthesis was restarted by adding MSAE in the culture repressed by actinomycin D. No revival of RSDG synthesis was observed by adding MSAE in the culture repressed by cycloheximide. MSAE improved RSDG production in A. niger by inducing the transcription of RSDG.
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