Abstract
Saccharomyces cerevisiae is a typical fermentation yeast in beer production. Improving ethanol tolerance of S. cerevisiae will increase fermentation efficiency, thereby reducing capital costs. Here, we found that S. cerevisiae strain L exhibited a higher ethanol tolerance (14%, v/v) than the fermentative strain Q (10%, v/v). In order to enhance the strain Q ethanol tolerance but preserve its fermentation property, protoplast fusion was performed with haploids from strain Q and L. The fusant Q/L-f2 with 14% ethanol tolerance was obtained. Meanwhile, the fermentation properties (flocculability, SO2 production, α-N assimilation rate, GSH production, etc.) of Q/L-f2 were similar to those of strain Q. Therefore, our works established a series of high ethanol-tolerant strains in beer production. Moreover, this demonstration of inactivated protoplast fusion in industrial S. cerevisiae strain opens many doors for yeast-based biotechnological applications.
Highlights
Beer has been consumed for long time all over the world
We found that S. cerevisiae strain L exhibited a higher ethanol tolerance (14%, v/v) than the fermentative strain Q (10%, v/v)
For strain Q and L, the log phase is from 8 h to 20 h, and the highest cell density is at 24 h, which is used as the moment for sampling
Summary
Beer has been consumed for long time all over the world. It is produced from barley by fermentation with yeast Saccharomyces cerevisiae, which is the best ethanol producer among many fermentative microorganisms. Erefore, strong improvement of yeast tolerance to high levels of ethanol would be beneficial for the cost-competitive bioethanol production. Creating yeast fusants by parental inactivated protoplast fusion, which the genomes of different strains were contained within one cell, to obtain yeast with superior ethanol tolerance was very significant in the beer industry. The fermentation properties (flocculability, SO2 production, α-N assimilation rate, GSH production, etc.) of Q/L-f2 were similar to those of strain Q. erefore, our works established one high ethanoltolerant strain in beer production This demonstration of inactivated protoplast fusion in industrial S. cerevisiae strain opens many doors for yeast-based biotechnological applications
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