Abstract
The effects of the genes of ORF53-55, which code ATP-binding cassette (ABC) transport proteins, on daptomycin production were investigated by overexpression. The yield of daptomycin was promoted significantly when either of the genes of ORF53, ORF54 or ORF55 was overexpressed individually or by a combined manner. The results above suggested that the ORF53, ORF54 and ORF55 genes had positive cooperativity in the biosynthesis of daptomycin. The dry weight and sugar utilization of HP-ORF53-55 is significantly improved as compared with the LC-54-16. Based on these results, it was speculated that the genes of ORF 53, 54 and 55 may play an important positive role in the process of reducing sugar transport, which enhanced the cell growth and daptomycin biosynthesis.
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