Improvement of cephalomanine production in Taxus chinensis cells by a combination of sucrose and methyl jasmonate

Abstract

Cell suspension cultures of Taxus chinensis, supplemented with 25 g sucrose l−1, produced 11 mg cephalomanine l−1, 21 g biomass l−1 and 19 nkat geranylgeranyl diphosphate (GGPP) synthase activity g protein−1. Supplementation of the cultures with 100 μM methyl jasmonate (MJA) produced 17 mg cephalomanine l−1, 6 g biomass l−1 and 78 nkat GGPP synthase activity g protein−1. Addition of sucrose and MJA together produced 24 mg cephalomanine l−1, 18 g biomass l−1 and 55 nkat GGPP synthase activity g protein−1.