Improvement of Candida parapsilosis by genome shuffling for the efficient production of arabitol from l-arabinose

Monika Kordowska-Wiater,Krzysztof Kostro,Urszula Lisiecka

doi:10.1007/s10068-018-0369-2

Monika Kordowska-Wiater, Krzysztof Kostro + Show 1 more

Open Access

https://doi.org/10.1007/s10068-018-0369-2

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Abstract

Arabitol is used in the food industry as a low-calorie sweetener. It is produced by yeasts during the biotransformation process of l-arabinose. Genome shuffling was performed in Candida parapsilosis DSM 70125, an efficient producer of arabitol, to obtain fusants with improved arabitol production ability. Four mutants from the parental library were used for the first round of genome shuffling. The best fusants, GSI-1 and GSI-10A, were subjected to a second round of genome shuffling. Finally, two fusants, GSII-3 and GSII-16, produced concentrations of arabitol that were 50% higher than that of the wild-type strain during selection culture. Under the optimal conditions established for C. parapsilosis, the two fusants produced 11.83 and 11.75 g/L of arabitol and were approximately 15–16% more efficient than the wild-type strain. Flow cytometry analysis showed that the ploidy of the new strains did not change.

Highlights

Genome shuffling is a strain improvement technology that combines classical mutagenesis with recombination
Genome shuffling was performed in Candida parapsilosis DSM 70125, an efficient producer of arabitol, to obtain fusants with improved arabitol production ability
Under the optimal conditions established for C. parapsilosis, the two fusants produced 11.83 and 11.75 g/L of arabitol and were approximately 15–16% more efficient than the wild-type strain

Summary

Introduction

Genome shuffling is a strain improvement technology that combines classical mutagenesis with recombination. Compared to other improvement techniques, genome shuffling has the advantage of exploiting the full genetic diversity of a population and offers the possibility of combining useful mutations from many different individuals [4] This engineering technique does not require expensive equipment and can be applied in every laboratory. Zhang and Geng [16] used a modified method of genome shuffling to combine S. cerevisiae cells with the P. stipitis genome to obtain isolates with better ethanol productivity. Another xylose-fermenting yeast, Pachysolen tannophilus, was genome shuffled to improve its tolerance to inhibitors in hardwood spent sulfite liquor [17]. To the best of the authors’ knowledge, this is the first report detailing genome shuffling in C. parapsilosis

Materials and methods

Results and discussion

Compliance with ethical standards