Improvement of biochar and bacterial powder addition on gaseous emission and bacterial community in pig manure compost

Abstract

Effect of bamboo biochar (BC) combined with two bacterial powders (B) on gaseous emission and variety of bacterial community during pig manure (PM) composting was investigated. The results showed that treatments of BC, BC + B1 and BC + B2 can reduce peak gaseous emission by 54%, 80% and 69% for CH4, respectively, while 37%, 45% and 45% for N2O, respectively, and 13%, 19% and 26% for NH3, respectively. The evolution of the bacterial community quantified with 16S rDNA analysis showed that in the thermophile stage, total relative abundance percentage of bacterial phyla of Firmicutes and Proteobacteria reached 97%, 97%, 93% and 96% for CK, BC, BC + B1 and BC + B2, respectively. Effects of BC on the compost bacterial community variation analysis proved bacterial activity in the thermophile stage was controlled by the content of dissolved organic carbon and temperature of the compost mixture, while electrical conductivity and total kjeldahl nitrogen also influenced compost maturity stage.