Abstract

We present a laser diode-based photoacoustic microscopy (PAM) system with a minimized light intensity loss for ovarian tissue imaging. A 905 nm, 650 W output peak power pulsed laser diode (PLD) is utilized as the light source. The intrinsic properties and the construction of this PLD typically make it challenging to focus its beam to a small spot size with a lowloss optical system. An optical system comprising a combination of aspheric and cylindrical lenses is presented that allows a low-loss collimation and tight focusing of the light beam. The lateral resolution of this PAM system is measured to be 40 μm using edge spread function estimation. Images of black human hairs, polyethylene tubes filled with rat blood, ex vivo mouse ear and ex vivo porcine ovary are presented.

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