Improved welan gum production by Alcaligenes sp. ATCC31555 from pretreated cane molasses

Abstract

Welan gum production by Alcaligenes sp. ATCC31555 from cane molasses was studied in batch fermentation to reduce production costs and enhance gum production. The pretreatment of cane molasses, agitation speed and the addition of supplements were investigated to optimize the process. Sulfuric acid hydrolysis was found to be the optimal pretreatment, resulting in a maximum gum concentration of 33.5g/L, which is 50.0% higher than those obtained from the molasses’ mother liquor. Agitation at 600rpm at 30°C and addition of 10% n-dodecane following fermentation for 36h increased the maximum gum production up to 41.0±1.41g/L, which is 49.1% higher than the greatest welan gum concentration in the literature so far. The welan gum product showed an acceptable molecular weight, similar rheological properties and better thermal stability to that obtained from glucose. These results indicate that cane molasses may be a suitable and inexpensive substrate for cost-effective industrial-scale welan gum production.