Abstract
Troughs made of metal or tape have been used to provide liquid reservoirs for ultrathin sectioning with glass knives (Hayat 1970). However, these types of trough have certain limitations. the metal trough is difficult to use and its paraffin seal is easily broken, causing leaks. Hayat(1970) has even suggested that fumes from heating this paraffin may be carcinogenic. the tape trough is easier to seal but deforms easily while sections are being collected and does not always provide an even surface for the observation of interference colors. This report describes a method of making inexpensive plastic troughs that can provide a reservoir comparable to those used with diamond knives.
Published Version
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