Improved tolerance toward low temperature in banana ( Musa AAA Group Cavendish Williams)

Abstract

To further understand the physiological mechanisms of cold-tolerance in banana plants, the responses of four introducing cultivars ( cv.) W811 (via long-term cold adaptation), PB, BJ10 and BJ11 to low-temperature stress (LT) were investigated. LT caused increased malondialdehyde (MDA) content, elevated contents of hydrogen peroxide (H 2O 2) and superoxide radical (O 2 −), and decreased photochemical efficiency ( F v/ F m) and net photosynthetic rate ( P n) in the leaves of four banana cultivars, but cv. W811 showed better LT tolerance than the other three cultivars. After 72 h of LT, four key antioxidative enzymes in the four cultivars showed different responses. Compared to controls, superoxide dismutase (SOD) activities in the four cultivars showed a significant decrease and W811 had the smallest amount of decrease. Catalase (CAT) activities showed a significant decrease. Peroxidase (POD) activities kept relatively higher activities and showed no significant changes (P > 0.05) in W811, BJ10 and BJ11 whereas that in PB showed a significant increase (P < 0.001). Ascorbate peroxidase (APX) activities in W811 and PB showed no significant changes (P > 0.05). Our results showed that higher cold-tolerance in cv. W811 may correlate with the long-term cold adaptation of the antioxidative enzymes such as SOD, POD and APX that alleviate oxidative stress caused by LT.