Abstract
INRA96 has been used successfully as a base extender for equine frozen semen [1-4]. The development of INRA96 [5] was a major step forward as an effective extender that used native phosphocaseinate. Independently, INRA96 (INRA96) was glycerolized [2, 3] and then augmented with the addition of egg-yolk [6, 7] to freeze stallion sperm cells. The objective of this research was to investigate the cryopreservation of stallion sperm using various percentages of glycerol and egg yolk. For the study, a wide range of stallion breeds (8) and ages (2 to 21 years) were used.
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