Abstract
Use of a convenient and inexpensive apparatus for trapping 14CO 2 from biological reactions in small volumes is described. Alternative scintillants for estimating 14CO 2 trapped in KOH are compared, toluene methoxyethanol (2:1) based scintillants and Instagel (TM-Packard) were efficient and more stable than the dioxane/Cab-O-Sil or toluene/Triton X-100 mixtures usually used. The usefulness of the technique is illustrated with microassays of OMP decarboxylase (EC 4.1.1.23), OMP pyrophosphorylase (EC 2.4.2.10) in the fission yeast Schizosaccharomyces pombe.
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