Abstract
Permeating the cell membrane with glycerol instead of detergents (e.g. NP‐40) and/or organic solvents (e.g. DMSO) significantly improves the visualization of F‐actin with rhodamine phalloidin. In particular, the thinnest bundles are more intensely stained, better delineated and observation time is prolonged dramatically. Furthermore, the method is gentle to membranous organelles, which makes, for example, co‐localization of mitochondria and actin possible.
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