Abstract

Garrison, W., Dale, A. and Saxena, P. K. 2013. Improved shoot multiplication and development in hybrid hazelnut nodal cultures by ethylenediamine di-2-hydroxy-phenylacetic acid (Fe-EDDHA). Can. J. Plant Sci. 93: 511-521. Micropropagation of hybrid hazelnut cultivars is difficult because of their recalcitrant nature. The current study assessed the effect of different iron sources on in vitro shoot multiplication and subsequent plantlet development from nodal explants of the cultivar Geneva. Two chelated forms of iron, ethylenediamine di-2-hydroxy-phenylacetic acid (Fe-EDDHA) and ethylenediamine tetraacetic acid (Fe-EDTA) were tested to determine the effect on shoot development. Shoots were longer and had a higher number of nodes when cultured on a modified NCGR-COR medium supplemented with 230 µM Fe-EDDHA, whereas shoots failed to grow on a medium with 460 or 690 µM Fe-EDTA. All plantlets grown in the presence of Fe-EDDHA had more chlorophyll, larger leaves, and higher dry weights compared with Fe-EDTA. El...

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