Abstract
Countercurrent chromatography (CCC) is liquid-liquid partition chromatography without using a solid support matrix. This technique requires further improvement of partition efficiency and shortening theseparation time. The locular multilayer coils modified with and without mixer glass beads were developed for the separation of proteins and 4-methylumbelliferyl (MU) sugar derivatives using the small-scale cross-axis coil planet centrifuge. Proteins were well separated from each other and the separation was improved at a low flow rate of the mobile phase. On the other hand, 4-MU sugar derivatives were sufficiently resolved with short separation time at a highflow rate of the mobile phase under satisfactory stationary phase retention. Effective separations were achieved using the locular multilayer coil for proteins with aqueous-aqueous polymer phase systems and for 4-MU sugar derivatives with organic-aqueous two-phase solvent systems by inserting a glass bead into each locule.
Highlights
Countercurrent chromatography (CCC) is liquid-liquid partition chromatography which eliminates the solid support matrix for stationary phase [1,2,3,4]
CCC is useful for separations mainly using organic-aqueous two-phase solvent systems [1,2,3,4], while the cross-axis CCC can be used for the separation of biopolymers with aqueous two-phase systems (ATPS) having low-interfacial tension and high viscosity
Insolvent order system to enhance mixing of theAstwo phases in each cross-axis CCC is useful for the separation of various compounds having a wide range of of the locular tubing, the extension of the compression length to increase the linear velocity of hydrophobicity from fatty acids to proteins with organic-aqueous and aqueous-aqueous two-phase the mobile phase and the insertion of a bead into each locule of the long-pressed locular tubing solvent systems, respectively
Summary
Countercurrent chromatography (CCC) is liquid-liquid partition chromatography which eliminates the solid support matrix for stationary phase [1,2,3,4]. The peak resolution and the stationary phase retention of this cross-axis depending on a new small-scale cross-axis CCC has been further developed in our laboratory to improve the the configuration of the separation column such as conventional multilayer coil [8,9,10], eccentric theoretical plates and peak resolution [8,9]. Insolvent order system to enhance mixing of theAstwo phases in each cross-axis CCC is useful for the separation of various compounds having a wide range of of the locular tubing, the extension of the compression length to increase the linear velocity of hydrophobicity from fatty acids to proteins with organic-aqueous and aqueous-aqueous two-phase the mobile phase and the insertion of a bead into each locule of the long-pressed locular tubing solvent systems, respectively.
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